Gene Expression and Regulation in Mammalian Cells

Gene Expression and Regulation in Mammalian Cells PDF Author: Fumiaki Uchiumi
Publisher: BoD – Books on Demand
ISBN: 9535138553
Category : Medical
Languages : en
Pages : 334

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Book Description
"Central dogma" was presented by Dr. Francis Crick 60 years ago. The information of nucleotide sequences on DNAs is transcribed into RNAs by RNA polymerases. We learned the mechanisms of how transcription determines function of proteins and behaviour of cells and even how it brings appearances of organisms. This book is intended for scientists and medical researchers especially who are interested in the relationships between transcription and human diseases. This volume consists of an introductory chapter and 14 chapters, divided into 4 parts. Each chapter is written by experts in the basic scientific field. A collection of articles presented by active and laboratory-based investigators provides recent advances and progresses in the field of transcriptional regulation in mammalian cells.

Gene Expression and Regulation in Mammalian Cells

Gene Expression and Regulation in Mammalian Cells PDF Author: Fumiaki Uchiumi
Publisher: BoD – Books on Demand
ISBN: 9535138677
Category : Medical
Languages : en
Pages : 372

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Book Description
Sixty years after the "central dogma," great achievements have been developed in molecular biology. We have also learned the important functions of noncoding RNAs and epigenetic regulations. More importantly, whole genome sequencing and transcriptome analyses enabled us to diagnose specific diseases. This book is not only intended for students and researchers working in laboratory but also physicians and pharmacists. This volume consists of 14 chapters, divided into 4 parts. Each chapter is written by experts investigating biological stresses, epigenetic regulation, and functions of transcription factors in human diseases. All articles presented in this volume by excellent investigators provide new insights into the studies in transcriptional control in mammalian cells and will inspire us to develop or establish novel therapeutics against human diseases.

Gene Expression and Regulation in Mammalian Cells - Transcription From General Aspects

Gene Expression and Regulation in Mammalian Cells - Transcription From General Aspects PDF Author: Fumiaki Uchiumi
Publisher:
ISBN: 9789535140955
Category : Genetics
Languages : en
Pages : 334

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Book Description
""Central dogma"" was presented by Dr. Francis Crick 60 years ago. The information of nucleotide sequences on DNAs is transcribed into RNAs by RNA polymerases. We learned the mechanisms of how transcription determines function of proteins and behaviour of cells and even how it brings appearances of organisms. This book is intended for scientists and medical researchers especially who are interested in the relationships between transcription and human diseases. This volume consists of an introductory chapter and 14 chapters, divided into 4 parts. Each chapter is written by experts in the basic scientific field. A collection of articles presented by active and laboratory-based investigators provides recent advances and progresses in the field of transcriptional regulation in mammalian cells.

Regulation of Gene Expression

Regulation of Gene Expression PDF Author: Gary H. Perdew
Publisher: Springer Science & Business Media
ISBN: 1597452289
Category : Science
Languages : en
Pages : 333

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Book Description
The use of molecular biology and biochemistry to study the regulation of gene expression has become a major feature of research in the biological sciences. Many excellent books and reviews exist that examine the experimental methodology employed in specific areas of molecular biology and regulation of gene expression. However, we have noticed a lack of books, especially textbooks, that provide an overview of the rationale and general experimental approaches used to examine chemically or disease-mediated alterations in gene expression in mammalian systems. For example, it has been difficult to find appropriate texts that examine specific experimental goals, such as proving that an increased level of mRNA for a given gene is attributable to an increase in transcription rates. Regulation of Gene Expression: Molecular Mechanisms is intended to serve as either a textbook for graduate students or as a basic reference for laboratory personnel. Indeed, we are using this book to teach a graduate-level class at The Pennsylvania State University. For more details about this class, please visit http://moltox. cas. psu. edu and select “Courses. ” The goal for our work is to provide an overview of the various methods and approaches to characterize possible mechanisms of gene regulation. Further, we have attempted to provide a framework for students to develop an understanding of how to determine the various mechanisms that lead to altered activity of a specific protein within a cell.

Gene Transfer and Expression in Mammalian Cells

Gene Transfer and Expression in Mammalian Cells PDF Author: S.C. Makrides
Publisher: Gulf Professional Publishing
ISBN: 9780444513700
Category : Science
Languages : en
Pages : 680

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Book Description
The production of proteins in mammalian cells is an important tool in numerous scientific and commercial areas. For example, proteins for human therapy, vaccination or diagnostic applications are typically produced in mammalian cells. Gene cloning, protein engineering, biochemical and biophysical characterization of proteins also require the use of gene expression in mammalian cells. Other applications in widespread use involve screening of libraries of chemical compounds in drug discovery, and the development of cell-based biosensors. This book presents a state-of-the-art comprehensive coverage of the technical aspects of gene expression in mammalian cells, written by experienced scientists working at the forefront of the field.

Eucaryotic Gene Regulation

Eucaryotic Gene Regulation PDF Author: Richard Axel
Publisher: Elsevier
ISBN: 0323143504
Category : Science
Languages : en
Pages : 682

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Book Description
Eukaryotic Gene Regulation covers the aspects and mechanisms of gene regulation of selected eukaryotes, such as yeast, Drosophila, and insect. This book is organized into eight parts, encompassing 52 chapters. The majority of the chapters are presented in an experimental manner containing an abstract, methods, results and discussion, and conclusion. This book first gives a short overview of the evolutionary role of interspersion in eukaryotic genes. It then presents considerable chapters on control of gene expression in yeast; gene mutation and isolation; structure and function; and analysis. Part III focuses on genetic and DNA sequence analysis in Drosophila. It includes discussions on allelic complementation and transvection, genetic organization, histone gene, and gene transcription. Part IV examines cell lineage; gene expression and sequences; and protein synthesis of insects, sea urchin, and mammalian cells. This is followed by discussions on structure and expression of specific eukaryotic genes from chicken, rat, rabbit, and human. Topics on the transfer of genetic information within and between cells and the structure and function of chromosome are significantly considered in Parts VI and VII. Genes evaluated in these sections include heavy chain immunoglobulin, light chain, beta-globin, and dihydrofolate reductase. Furthermore, this book describes the in vitro transcription and the factors involved; internal organization and mechanism of assembly of nucleosome; and chromatin structure. The concluding section focuses on aspects of viral genome expression including gene regulation, synthesis, processing, and alternative RNA splicing. Research biologists, geneticists, scientists, teachers, and students will greatly benefit from this book.

Cell Cycle Regulation

Cell Cycle Regulation PDF Author: James R. Jr. Jeter
Publisher: Elsevier
ISBN: 0323158161
Category : Science
Languages : en
Pages : 276

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Book Description
Cell Cycle Regulation describes the interaction of the nuclear genome, the cytoplasmic pools, the organelles, the cell surface, and the extracellular environment that govern the cell cycle regulation. Comprised of 12 chapters, this book includes cell cycle regulation around nuclear chromatin modulation and some aspects of chromatin modification and its effects on gene expression. The opening chapters describe the macromolecular structure of chromatin subunits and the types and kinds of postsynthetic modifications occurring on histones, such as acetylation, methylation, and phosphorylation. The subsequent chapter deals extensively on histone phosphorylation, especially histone H1, H1M, H2A, and H3, during the cell cycle. Another chapter describes a selective histone leakage from nuclei during isolation accounting for the role of histone acetylation and phosphorylation in gene expression. This book goes on examining the assembly of microtubules and structural analysis on the regulatory role of calcium into a pattern for mitosis regulation. Other chapters discuss the methods used to measure intracellular pH changes as a function of the cell cycle of Physarum and the quantitative and qualitative changes taking place during the various phases of the cell cycle. The use of mammalian cell fusion to study cell cycle regulation and the protein synthesis regulation during the cell cycle in Chlamydomonas reinhardi are then discussed. The final chapters focus on the regulation of expression of an inducible structural gene during the cell cycle of the green alga Chlorella. The chapters provide evidence for a model of positive and negative oscillatory control of inducible gene expression. An analysis of the expression of cytoplasmic genes as a function of the cell cycle using pedigrees of a large number of individual yeast cells is also included. This book will appeal to a wide variety of life scientists and to molecular, cellular, and developmental biologists.

Gene Expression and Regulation in Mammalian Cells

Gene Expression and Regulation in Mammalian Cells PDF Author: Fumiaki Uchiumi
Publisher: BoD – Books on Demand
ISBN: 9535138553
Category : Medical
Languages : en
Pages : 334

View

Book Description
"Central dogma" was presented by Dr. Francis Crick 60 years ago. The information of nucleotide sequences on DNAs is transcribed into RNAs by RNA polymerases. We learned the mechanisms of how transcription determines function of proteins and behaviour of cells and even how it brings appearances of organisms. This book is intended for scientists and medical researchers especially who are interested in the relationships between transcription and human diseases. This volume consists of an introductory chapter and 14 chapters, divided into 4 parts. Each chapter is written by experts in the basic scientific field. A collection of articles presented by active and laboratory-based investigators provides recent advances and progresses in the field of transcriptional regulation in mammalian cells.

Gene Expression and Regulation in Mammalian Cells

Gene Expression and Regulation in Mammalian Cells PDF Author: Fumiaki Uchiumi
Publisher: BoD – Books on Demand
ISBN: 9535138677
Category : Medical
Languages : en
Pages : 372

View

Book Description
Sixty years after the "central dogma," great achievements have been developed in molecular biology. We have also learned the important functions of noncoding RNAs and epigenetic regulations. More importantly, whole genome sequencing and transcriptome analyses enabled us to diagnose specific diseases. This book is not only intended for students and researchers working in laboratory but also physicians and pharmacists. This volume consists of 14 chapters, divided into 4 parts. Each chapter is written by experts investigating biological stresses, epigenetic regulation, and functions of transcription factors in human diseases. All articles presented in this volume by excellent investigators provide new insights into the studies in transcriptional control in mammalian cells and will inspire us to develop or establish novel therapeutics against human diseases.

Gene Expression and Regulation in Mammalian Cells - Transcription From General Aspects

Gene Expression and Regulation in Mammalian Cells - Transcription From General Aspects PDF Author: Fumiaki Uchiumi
Publisher:
ISBN: 9789535140955
Category : Genetics
Languages : en
Pages : 334

View

Book Description
""Central dogma"" was presented by Dr. Francis Crick 60 years ago. The information of nucleotide sequences on DNAs is transcribed into RNAs by RNA polymerases. We learned the mechanisms of how transcription determines function of proteins and behaviour of cells and even how it brings appearances of organisms. This book is intended for scientists and medical researchers especially who are interested in the relationships between transcription and human diseases. This volume consists of an introductory chapter and 14 chapters, divided into 4 parts. Each chapter is written by experts in the basic scientific field. A collection of articles presented by active and laboratory-based investigators provides recent advances and progresses in the field of transcriptional regulation in mammalian cells.

Gene Expression and Regulation in Mammalian Cells - Transcription Toward the Establishment of Novel Therapeutics

Gene Expression and Regulation in Mammalian Cells - Transcription Toward the Establishment of Novel Therapeutics PDF Author:
Publisher:
ISBN: 9789535138686
Category :
Languages : en
Pages :

View

Book Description


Regulation of Gene Expression

Regulation of Gene Expression PDF Author: Gary H. Perdew
Publisher: Springer Science & Business Media
ISBN: 1597452289
Category : Science
Languages : en
Pages : 333

View

Book Description
The use of molecular biology and biochemistry to study the regulation of gene expression has become a major feature of research in the biological sciences. Many excellent books and reviews exist that examine the experimental methodology employed in specific areas of molecular biology and regulation of gene expression. However, we have noticed a lack of books, especially textbooks, that provide an overview of the rationale and general experimental approaches used to examine chemically or disease-mediated alterations in gene expression in mammalian systems. For example, it has been difficult to find appropriate texts that examine specific experimental goals, such as proving that an increased level of mRNA for a given gene is attributable to an increase in transcription rates. Regulation of Gene Expression: Molecular Mechanisms is intended to serve as either a textbook for graduate students or as a basic reference for laboratory personnel. Indeed, we are using this book to teach a graduate-level class at The Pennsylvania State University. For more details about this class, please visit http://moltox. cas. psu. edu and select “Courses. ” The goal for our work is to provide an overview of the various methods and approaches to characterize possible mechanisms of gene regulation. Further, we have attempted to provide a framework for students to develop an understanding of how to determine the various mechanisms that lead to altered activity of a specific protein within a cell.

Gene Transfer and Expression in Mammalian Cells

Gene Transfer and Expression in Mammalian Cells PDF Author: S.C. Makrides
Publisher: Gulf Professional Publishing
ISBN: 9780444513700
Category : Science
Languages : en
Pages : 680

View

Book Description
The production of proteins in mammalian cells is an important tool in numerous scientific and commercial areas. For example, proteins for human therapy, vaccination or diagnostic applications are typically produced in mammalian cells. Gene cloning, protein engineering, biochemical and biophysical characterization of proteins also require the use of gene expression in mammalian cells. Other applications in widespread use involve screening of libraries of chemical compounds in drug discovery, and the development of cell-based biosensors. This book presents a state-of-the-art comprehensive coverage of the technical aspects of gene expression in mammalian cells, written by experienced scientists working at the forefront of the field.

Regulation of Gene Expression in Eukaryotic Cells

Regulation of Gene Expression in Eukaryotic Cells PDF Author: Maureen I. Harris
Publisher:
ISBN:
Category : Biochemical genetics
Languages : en
Pages : 127

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Book Description


The Regulation of Tissue-specific Gene Expression in Mammalian Cells in Culture

The Regulation of Tissue-specific Gene Expression in Mammalian Cells in Culture PDF Author: A. A. Farmer
Publisher:
ISBN:
Category : Gene expression
Languages : en
Pages : 362

View

Book Description


Eucaryotic Gene Regulation

Eucaryotic Gene Regulation PDF Author: Richard Axel
Publisher: Elsevier
ISBN: 0323143504
Category : Science
Languages : en
Pages : 682

View

Book Description
Eukaryotic Gene Regulation covers the aspects and mechanisms of gene regulation of selected eukaryotes, such as yeast, Drosophila, and insect. This book is organized into eight parts, encompassing 52 chapters. The majority of the chapters are presented in an experimental manner containing an abstract, methods, results and discussion, and conclusion. This book first gives a short overview of the evolutionary role of interspersion in eukaryotic genes. It then presents considerable chapters on control of gene expression in yeast; gene mutation and isolation; structure and function; and analysis. Part III focuses on genetic and DNA sequence analysis in Drosophila. It includes discussions on allelic complementation and transvection, genetic organization, histone gene, and gene transcription. Part IV examines cell lineage; gene expression and sequences; and protein synthesis of insects, sea urchin, and mammalian cells. This is followed by discussions on structure and expression of specific eukaryotic genes from chicken, rat, rabbit, and human. Topics on the transfer of genetic information within and between cells and the structure and function of chromosome are significantly considered in Parts VI and VII. Genes evaluated in these sections include heavy chain immunoglobulin, light chain, beta-globin, and dihydrofolate reductase. Furthermore, this book describes the in vitro transcription and the factors involved; internal organization and mechanism of assembly of nucleosome; and chromatin structure. The concluding section focuses on aspects of viral genome expression including gene regulation, synthesis, processing, and alternative RNA splicing. Research biologists, geneticists, scientists, teachers, and students will greatly benefit from this book.

Cell Cycle Regulation

Cell Cycle Regulation PDF Author: James R. Jr. Jeter
Publisher: Elsevier
ISBN: 0323158161
Category : Science
Languages : en
Pages : 276

View

Book Description
Cell Cycle Regulation describes the interaction of the nuclear genome, the cytoplasmic pools, the organelles, the cell surface, and the extracellular environment that govern the cell cycle regulation. Comprised of 12 chapters, this book includes cell cycle regulation around nuclear chromatin modulation and some aspects of chromatin modification and its effects on gene expression. The opening chapters describe the macromolecular structure of chromatin subunits and the types and kinds of postsynthetic modifications occurring on histones, such as acetylation, methylation, and phosphorylation. The subsequent chapter deals extensively on histone phosphorylation, especially histone H1, H1M, H2A, and H3, during the cell cycle. Another chapter describes a selective histone leakage from nuclei during isolation accounting for the role of histone acetylation and phosphorylation in gene expression. This book goes on examining the assembly of microtubules and structural analysis on the regulatory role of calcium into a pattern for mitosis regulation. Other chapters discuss the methods used to measure intracellular pH changes as a function of the cell cycle of Physarum and the quantitative and qualitative changes taking place during the various phases of the cell cycle. The use of mammalian cell fusion to study cell cycle regulation and the protein synthesis regulation during the cell cycle in Chlamydomonas reinhardi are then discussed. The final chapters focus on the regulation of expression of an inducible structural gene during the cell cycle of the green alga Chlorella. The chapters provide evidence for a model of positive and negative oscillatory control of inducible gene expression. An analysis of the expression of cytoplasmic genes as a function of the cell cycle using pedigrees of a large number of individual yeast cells is also included. This book will appeal to a wide variety of life scientists and to molecular, cellular, and developmental biologists.